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They are all quite dark, so under exposure could easily be doing that. With negative film, err on the side of over exposure, with Portra 400, don't be afraid to over expose by *many* stops, it'll be fine. Your flatbed is not optimal for 35mm negs, but you should still be able to get better than that. Perhaps let Vuescan have multiple passes at the neg, see if it can drag some detail out. Also, try sharpening or noise reduction in Lightroom, Neat Image, something like that.
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